Establishment of heterotopic cardiac transplantation model in mice

Objective To establish a mouse model of heterotopic heart transplantation and construct an animal model for the study of transplantation immunity.Methods According to the surgical procedures of Ono's pattern in mice cardiac heterotopic transplantation model,50 Kunming mice were divided into donors and recipients randomly.The donor heart aorta and the recipient ventral aorta,the donor pulmonary artery and the recipient inferior caval vein,were anastomosed by using the end-to-side suture technique respectively.Results The mouse model of heterotopic heart transplantation was established successfully with a success rate of 84％ (21/25).The average time of donor operation and recipient operation was (11.0 ± 1.0) min and(60.0 ± 2.0) min respectively.Conclusions In the base of mastering the technique of operation facility,the model of mice heart heterotopic transplantation would be established successfully. Key words: Mice ;  Abdomen;  Heart transplantation ;  Models