Inhibitory Effect of Extract Granule of Earthworms (Lumbricus rubellus) on the Pathogenic Bacteria In Vitro

The objective of this study was to determine the inhibition ability of the earthworm ( L. rubellus ) extract (ECT), dried earthworm extract (ECT-k), and granule earthworm extract (ECT-g) as poultry feed additive against some pathogenic bacteria. Antibacterial activity was performed using diffusion method against Escherichia coli , Salmonella pullorum, Pseudomonas aeruginosa , and Staphylococcus aureus  in vitro . In the present study, the concentrations of ECT, ECT-k, and ECT-g in nutrient broth (NB) media tested were consisted of treatments A: 0%, B: 0.26%, C: 0.52%, D: 0.78% and E: 1.04% (g/vol) respectively. The results of the in vitro study showed that started from ECT level 0.26% inhibited (P 0.05) of ECT and ECT-t against S. pullorum. Diameter of inhibition zone for 24 hours showed that S. aureus was the most sensitive bacterium to ECT and ECT-k, and S. pullorum was the most sensitive bacterium to ECT-g.