Characterization of a Nutritive Synchrony in L-cells

Summary A synchronization method in L-cells producing nutritive stagnation of the cells in G 1 is presented. After reinnoculation, cells proceed through the cycle in the normal 24 hours. The method has the advantage that no chemical blockers are added to the system and a large number of cells is obtained. In a comparison with other synchronizing systems, this system rates in the foreground. Desynchronization due to individual cell physiologies makes investigations of G 2 difficult with this system. The method is uncomplicated and probably applicable to similar cell types.