Hybridization of DNA to Bead-Immobilized Probes Confined within a Microfluidic Channel

We report the factors influencing the capture of DNA by DNA-modified microbeads confined within a microfluidic channel. Quantitative correlation of target capture efficiency to probe surface concentration, solution flow rate, and target concentration are discussed. The results indicate that the microfluidic system exhibits a limit of detection of ∼10-10 M (∼10-16 mol) DNA and a selectivity factor of ∼8 × 103. Typical hybridization times are on the order of minutes.