Preparation of whole-fiber cotton gel-filtration chromatography columns

Abstract Methods of preparing whole-fiber gel-filtration columns are described. Reproducible results were obtained with columns prepared by packing with randomized fiber mat type discs (such as cut from absorbent cotton mat rolls). Results from these columns compared favorably with Sephadex columns. The columns had a useful life exceeding one year. Techniques for reducing void volume and increasing the accessible pore volume are described.