Establishment of Rapid Adventitious Bud Induction from Mature Zygotic Embryos of Birch(Betula platyphylla Suk.)

Rapid adventitious bud induction of Betula platyphylla was establinshed from mature zygotic embryos.After disinfect,the zygotic embryos were cut vertically,and induced adventitious buds directly.In the media of WPM+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,the rate of adventitious buds induction was 93.3%,and number of adventitious buds was 8.7.In the media of WPM+4.0 mg·L-1 TDZ,the rate was 90.2%,and the number was 7.6.These results indicated that WPM+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA was better for induction of adventitious buds.And NAA could promote the rooting of adventitious shoots,the optimum concentration was 0.2 mg·L-1.