Isolating Cells from Tissue

This chapter will briefly touch on the history of cell theory, followed by an overview of main intracellular components. This is followed by a discussion as to why, depending on their primary function, mature cells differ so much in the shapes and intracellular content. We next focus on the methods to isolate and sort different cell types. Main types of centrifugation and differences between them are then introduced, followed by an overview of how cells can be counted and evaluated for their viability after isolation.