Nuclear localization of Kaiso as a prognostic biomarker and promotion of EMT in infiltrating ductal breast carcinomas.

e22061 Background: The expression and biological consequences of Kaiso, a novel BTB-POZ bi-modal transcription factor, in infiltrating ductal carcinomas (IDCs) have not been widely investigated. Methods: Kaiso expression and subcellular localization was determined in 146 normal tissues, 376 IDCs, and 85 lymph node metastases by immunohistochemistry. Semi-quantitative analysis of staining was evaluated with clinicopathological features of IDCs using Chi-square analysis and Kaplan Meier Curves. MCF-7 cells were induced to overexpression Kaiso, whereas MDA-MB-468 and MDA-MB-231 cells were treated with si-Kaiso or si-Scr and assayed for cell migration, invasion, RNA and protein expression. Chromatin Immunoprecipitation assay was performed in MDA-MB-231 cells to determine the direct interaction of Kaiso with methylated sequences within E-cadherin promoter. Results: Kaiso expression in both the cytoplasmic and nuclear compartments correlated with age <48 (cytoplasmic p<0.0093; nuclear p< 0.0001) and moderate di...