RNA Extraction and Preparation in Rice (Oryza sativa)

There has been a surge of interest in the molecular biology of RNA. We describe a simple, fast, and convenient protocol for isolating total RNA from rice (Oryza sativa) seedlings and young tissues/organs. RNA obtained using this method can be used for reverse transcription-polymerase chain reaction (RT-PCR), quantitative RT-PCR (qRT-PCR), northern blot analysis, RNA-seq, microarray analysis, cDNA library construction, etc. Technical parameters critical to the success of the extraction are presented. © 2016 by John Wiley & Sons, Inc. Keywords: rice; RNA isolation; total RNA