Генетические маркеры вакцинных штаммов метапневмовируса птиц

Virulent and attenuated strains of avian metapneumovirus (aMPV) distinguished by a number of genetic signs, thorough study acquired a special meaning for selection of natural-attenuated and vaccine strains that used for the specific prophylaxis of disease. It has been conducted the study of genetic markers of the production strains 8544 and VC-03 aMPV, are associated with features of their replication and identified in vitro. It has been showed, that the strains of the virus are induced the acute form of virus infection in the primary cell cultures with different latent period depending on the type of culture. Their maximal accumulation was found in Vero cells with sowing concentration 2×105 cells/cm3 by inoculation of monolayer after 48–72 hours and the time of virus removal after 72 hours after the inoculation. It has been confirmed the possibility of using the tracheal organ culture for the isolation and study of aMPV markers. It has been installed the ability of strains 8544 and VC-03 to the replication in Vero cells by 34 and 40 °C and induction of interferon, which concentration was in direct dependency of strain-inductor multiplicity. Strains of virus are susceptible to the effect of exogenous interferon.