FUNDAÇÃO OSWALDO CRUZ FIOCRUZ CENTRO DE PESQUISA AGGEU MAGALHÃES MESTRADO EM BIOCIÊNCIAS E BIOTECNOLOGIA EM SAÚDE

Juvenile systemic lupus erythematosus (JSLE) is a chronic inflammatory disease caused by the production of autoantibodies that recognize self-antigens as foreign, and have high morbidity and mortality in relation to the same disease in adults. The pathogenesis of SLE involving environment, hormonal, imunologics and genetics factors. Genetic association studies demonstrated that susceptibility to lupus is polygenic, and between the genes involved is HLA-G which is responsible for the regulation of the immune response. In the study, we analyzed the isoform expression of HLA-G molecule and observed the immune response profile in patients with juvenile systemic lupus erythematosus. Patients in the study were selected at the Hospital of Rheumatology Clinics service and peripheral blood was collected from 10 patients in the active and inactive time of JSLE. The study compared the expression of HLA-G5, IL-10 and TNF mRNA, plasma levels of sHLA-G and Th1 Th2 and Th17 cytokines between groups with the disease active and inactive and the group of healthy children by applying the test Mann-Whitney U test and Kruskal-Wallis. The results showed that patients with active lupus high levels of IL-10 (p=0,004) and IL-6 (p=0,011), besides presenting mRNA expression TNF higher than the inactive group (p <0.05). Even with few, we could characterize that there was a change of cytokine profile during activation of JSLE,and the ratio of activation with soluble HLA-G expression in addition to serving as a basis for proposing a regulation model immune, which can be validated in future experiments.