Optical clearing and multimodality fluorescence and magnetic resonance imaging in cancer models

Multimodality registration of optical and magnetic resonance imaging (MRI) signals in the same voxels of live tissue could be useful for improving accuracy of optical image reconstruction. We investigated OC effects on fluorescence intensity (FI) imaging of red fluorescent TagRFP protein marker in tumor cells and combined it MRI. The OC effects of diamagnetic glycerol/DMSO/water and a paramagnetic magnetic resonance (MR) imaging agent (gadobutrol) and its mixtures were measured by using whole body FI, a single-photon counting FI setup and three MRI pulse sequences. The obtained results indicate that MRI is important modality enabling mechanistic studies of OC effects in the skin and peripheral subcutaneous tissue.