Crystallization and preliminary crystallographic analysis of [A21Ser-B27Arg-B30Thr-NH 2 ]-human insulin

Some experiments have shown that the properties of single mutation can be synthesizedin a mutant with multiple mutations. Following this idea, SRN-HI was prepared.Markussen et al.reported that a high stability and a prolonged action could be createdfrom mutations AsnA21Ser and ThrB27Arg respectively. Therefore, a double property wasexpected to appear in SRN-HI, which has been proved to be true by biological test.