Approach to Serial Liquid Biopsy: Enrichment of circulating tumor cells (CTC) in breast cancer patients for cancer panel analysis.

e22029 Background: Although many effective therapies improve the survival rate in breast cancer patients, a significant number of patients could not avoid recurrence and metastasis. Liquid biopsy using circulating tumor cells (CTC) is a non-invasive method to obtain tumor cells, and can be a substitute for tumor tissue biopsy. This new approach may provide molecular profiling of tumor, which can be useful for determination of personalized cancer therapy according to the molecular profiling of individual patient during the process of tumor diagnosis, recurrence, and metastasis. Methods: Blood samples (10 ml) were collected, and processed through Cytogen protocol to enrich CTCs. In brief, whole blood was incubated with specific antibodies against RBC and WBC, and spun down followed passing through micro fabricated porous filter (CytoGen). Enriched CTCs were divided into two parts, one for counting and one for molecular analysis. For CTC counting, cells were stained for EpCAM, CD45, and DAPI. DNAs were extra...