フロー式反応装置による umu test の自動化にともなう測定条件

Various bioassays have been applied to evaluate safety of environmental water and treated water.The umu test is a method for the detection of genotoxicity by using SOS function of Salmonella typhimurium.The protocol of the nmu test is divided into 5 phases, (1) pre-incubation of test strain, (2) addition of samples, (3) induction of umuC gene, (4) β-galactosidase enzymatic reaction, and (5) measurement of absorption in a spectrophotometry and calculation of the activity.In usual procedure, all phases are performed in batch-type reactors, using test tubes.In this study, we tried to develop the device of flow-injection style to perform the umu test automatically. The culture was flowed through a tube and added samples by using a rotary pump. The various conditions were investigated and modified for detection of genotoxicity of AF-2.Even after 24hr of incubation under control, test strain showed activity. Within 1.5hr, the test strain was most active at 36°C and longer than 2.0hr, it was so at 37°C.At each phases some more conditions have been investigated, and we suggest that new device of flow-injection style could be effective to screen or monitor the genotoxicity of environmental water.