Community monitoring method of microorganism carried by air particulate matters with multiple particle sizes

The invention discloses a community monitoring method of microorganism carried by air particulate matters with multiple particle sizes. The method comprises the following steps: (1) preparing an instrument; (2) collecting a sample; (3) transferring the sample; (4) extracting DNA; (5) carrying out PCR amplification; (6) carrying out high-throughput sequencing; (7) analyzing the community; and (8) establishing a database. The method can be used for researching the community structure of the microorganism carried by atmospheric particulates under any different meteorological conditions and analyzing the microorganism carried on the surfaces of multiple particulates such as TSP, PM10 and PM2.5; compared with the traditional cultivation analysis method, the community monitoring method has the advantages of wide coverage, high detection accuracy and sensitivity and the like in the aspect of atmospheric microbial community detection, thus having an important value in the aspects of evaluating the influence of air microorganism in the haze to the human health, judging the production source of the haze, and the like.