Enzymatic Lipophilization of d-Borneol Extracted from Cinnamomum camphora chvar. Borneol Seed

2021 
Abstract Borneol esters have attracted increasing interest due to their potential application in the food industry. In this study, the extraction of d-borneol from Cinnamomum camphora chvar. borneol seed was carried out via steam distillation to gain highly purified d-borneol (99.66%). The lipase-catalyzed lipophilization of extracted d-borneol with oleic acid was performed in a solvent-free system. The influences of lipase type, substrate molar ratio, lipase loading, reaction temperature and reaction time on d-bornyl oleate yield were evaluated. Among four kinds of commercial lipases screened for their esterification efficiency, CRL (lipase from Candida rugose) displayed the highest catalytic activity in the synthesis of d-bornyl oleate. The yield of more than 95% was obtained under the optimal conditions: substrate molar ratio 1:2 (d-borneol/oleic acid), lipase loading 8%, reaction temperature 45 °C, reaction time 48 h. The structural identification of d-borneol and product d-bornyl oleate was performed by gas chromatography mass spectrometry (GC-MS) and proton nuclear magnetic resonance spectroscopy (1H NMR). The results showed that the product was confirmed to be d-bornyl oleate and the purity of which was 97.51%. This study will pave the way for d-borneol and its esters application in food industry.
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