Screening of High Pristinamycin-Producing Strain by Combined Mutation

After the spore suspension of Streptomyces pristinaespiralis ZP2 was treated by combined mutation with UV and NTG,the pristinamycin resistant mutants were screened,and a pristinamycin resistant mutant UN2056 was obtained.The product of mutant UN2056 reached 1490 mg/L,which was 45.7% more than that of the parent strain ZP2.The subculture experiments indicated that the hereditary character of high productivity of S.pristinaespiralis UN2056 was stable.The production of mutant UN2056 in 5L fermentor reached 1645 mg/L,which was 60.8% more than that of the parent strain.