POLYACETYLENE AND CAROTENES FROM PETROSELINUM SATIVUM ROOT

Polyacetylenes, carotenes and other components such as starch, pectin, cellulose, lignin were investigated in the root of Petroselinum sativum by a noninvasive spectroscopic method. The components were measured in situ, directly in the plant tissue, with no preliminary sample preparation. The analysis was done on the basis of intensity of characteristic bands observed in Raman spectrum. The principal polyacetylenes from P. sativum are all–cis–polyacetylene (C2H2)n and all–trans–polyacetylene (C2H2)n have similar molecular structure, but present in their spectra a change of the symmetric group – C=C–C=C– from 2210 cm–¹ to 2002 cm–¹. The differences observed can be due both to the conformational differences and those existing in the environment. By Raman quantification were detected polyacetylenes that visualize the distribution of biostructures between the sections in root of P. sativum. The technique of quantification was applied to evaluate the distribution of some compounds such as lignin and polyglucides. Results showed an internal tissue specific accumulation in starch and biostructures like lignin, pectin and cellulose in the cell wall.