Isolation and characterization of microsatellite loci for the endangered Midwife Betic toad Alytes dickhilleni (Discoglossidae)

We have developed eleven polymorphic microsatellite loci for the amphibian Alytes dickhilleni using an enriched-library approach. We detected 98 alleles in 50 individuals genotyped (mean number of alleles per locus was 8.91) in two different populations in South East Spain. Expected heterozygosities ranged from 0.324 to 0.891 in one population (Arroyo Guadahornillos) and 0.424–0.909 in the other population (Cueva Paria). The levels of polymorphism of the developed markers render them readily applicable for population genetic studies of diversity, structure, and migration.