[Effects of adipose-derived stem cells and endothelial cells on survival and neovascularization of fat tissue transplants].

目的: 探讨脂肪来源干细胞（adipose-derived stem cells，ADSCs）及血管内皮细胞（endothelial cells，ECs）对脂肪移植术后血管新生及脂肪成活的影响。. Methods: The ADSCs were isolated by collagenase digestion from the adipose tissues voluntarily donated by the patients undergoing mastectomy, and subcultured. The passage 3 ADSCs were used for subsequent experiments. The residual fat tissues were used to prepare fat particles (FPs). The human umbilical vein endothelial cells (HUVECs) were used as ECs for subsequent experiments. Eighty healthy male nude mice, aged 4-6 weeks, were randomly divided into 4 groups ( n=20). The mice were received subcutaneous injection at the dorsum of 1 mL FPs+0.3 mL normal saline (NS) in control group, 1 mL FPs+2×10 6 ECs+0.3 mL NS in ECs group, 1 mL FPs+2×10 6 ADSCs+0.3 mL NS in ADSCs group, and 1 mL FPs+1×10 6 ECs+1×10 6 ADSCs+0.3 NS in ADSCs+ECs group. General observations of the injection sites were performed, and the survival of the mice was recorded. At 2, 4, 8, and 12 weeks after injection, grafted fat tissues were firstly assessed by ultrasonography, then they were collected for volume measurement (water displacement method) and histology observation (HE staining and immunofluorescence staining). 结果: 各组裸鼠均存活至实验完成。移植后各时间点各组超声检测结果无明显差异，移植脂肪内部均未见明显血流信号，未见囊肿、钙化、实性占位等异常表现。移植后各时间点，ADSCs 组及 ADSCs+ECs 组移植脂肪体积均高于 ECs 组及对照组（ P 0.05）。. 结论: ADSCs 可提高移植脂肪存活率，且该促进作用可能与促进血管新生相关。.