Prognostic investigations of B7-H1 and B7-H4 expression levels as independent predictor markers of renal cell carcinoma.

Abstract In order to evaluate the correlation of B7-H4 andB7-H1 with renal cell carcinoma (RCC), we analyzed B7-H1and B7-H4 expressions and their clinical significance by immunohistochemicalmethod. Our result indicated that B7-H4-positive staining was detected in 58.13 % of RCC tissues (25tissues tumors), and there were 18 tissues of patients withoutdetectable B7-H4. Furthermore, 21 cases (48.83 %) were B7-H1-positive. Positive tumor expressions of B7-H4 and B7-H1were markedly related to advanced TNM stage (P= 0.001;P= 0.014), high grade (P=0.001; P= 002), and larger tumorsize (P=0.002; P= 024) in RCC tissues than patients with B7-H4-negative and B7-H1-negative in RCC tissues. The patientswith B7-H1 and B7-H4-positive expressions were found to bemarkedly correlated with the overall survival of the patients(P< 0.05) and tended to have an increased risk of death whencompared with negative expression groups. Univariateanalysis showed that B7-H4 and B7-H1 expressions, TNMstage, high grade, and tumor size were significantly relatedto the prognosis of RCC. Furthermore, multivariate analysisshowed that B7-H4 and B7-H1 expressions decreased overallsurvival. The adjusted HR for B7-H1 was 2.83 (95 % CI1.210–2.971; P = 0.031) and also was 2.918 (95 % CI1.243–3.102; P= 0.006) for B7-H4 that showed these markerswere independent prognostic factors in RCC patients. Theexpressions of B7-H1 and B7-H4 in RCC patients indicatethat these markers may be as a predictor of tumor developmentand death risk. Further investigations can be helpful toconfirm B7-H1 and B7-H4 roles as an independent predictorof clinical RCC outcome.Keywords B7-H1 and B7-H4 . Renal cell carcinoma .Analysis . Prognosis . Immunohistochemistry