Isolation and characterization of oil-degrading marine micro-organisms

By enriching with diesel fuel as a sole carbon source, two microbial consortia were obtained from different marine environment samples: one, colleted from the Huanghe Dock of Shengli Oil Field, and the other, from the Oil storage Dock Xiamen. Nine bacteria and one fungus were screened out from the two samples. All of the isolates show considerable biodegradability to diesel fuel except for M-5, while it shows degradability to several kinds of PAHs. Sequence analysis of 16S rDNA identifies that the 9 bacteria belong to Bacillus cereus, Pseudomonas stutzeri, Staphylococcus arlettae, Alcanivorax sp., Alcaligenes denitrificans and alpha proteobacterium (isolate M-5), respectively. Among them, each of isolates B-5 and M-5 belongs to a novel species. Two alkane monoxygenase fragments ware amplified from isolate B-5 and Pseudomonas stutzeri M-2. And the fragment retrieved from isolate B-5 seems to encode a partial novel alkane monoxygenase.