Studies on the Fermentation Conditions of Sphaerotilus natans FQ40 Biosynthesis Poly-β-hydroxybutyrate(PHB)

从活性污泥中分离筛选到1株产PHB的球衣菌FQ40.其最适发酵培养基配方为(g/L):蔗糖10,牛肉膏5,MgSO4·7H2O 0.2,CaCl2 0.05,FeCl3 0.01,K2HPO4 0.04,KH2PO4 0.03,NaH2PO4·2H2O 0.05,H3BO3 0.005.最佳摇瓶发酵条件为:250mL三角瓶装80mL培养液,起始pH为7.0,培养温度30℃,接种量10%,转速150r/min,周期42h.在优化条件下,细胞干重达4.44g/L,PHB含量为48%,PHB浓度为2.13g/L.