Study on isolation and biological characteristics of Lactobacillus in intestines of pig.

Objective To separate lactic acid bacteria from pig digestive tract.Method The fresh stool samples were collected from healthy piglets and inoculated in MRS culture medium to isolate lactobacillus by using anaerobic culture.The isolates were investigated according to biological characteristic tests such as their morphology,biochemical characters,lactic acid production,acid resistance,tolerance to bile salts,high temperature tolerance and antagonistic property.Result Five strains of Lactobacillus acidophilus were isolated.Culture supernatants of the five strains showed different degrees of antagonistic effect against pathogenic E.coli and Streptococcus.Conclusion It lays a foundation for developing highly efficient and specific probiotics for pigs.