The chemokine CCL5 as a potential prognostic factor predicting disease progression in stage II breast cancer patients.

Purpose: The aim of this study was to determine the prognostic value of the chemokine CCL5, considered as a promalignancy factor in breast cancer, in predicting breast cancer progression and to evaluate its ability to strengthen the prognostic significance of other biomarkers. Experimental Design: The expression of CCL5, alone and in conjunction with estrogen receptor (ER)-a ,E R-h, progesterone receptor (PR), and HER-2/neu (ErbB2), was determined in breast tumor cells by immunohistochemistry. The study included 142 breast cancer patients, including individuals in whom disease has progressed. Results: Using Cox proportional hazard models, univariate analysis suggested that, in stage I breast cancer patients, CCL5 was not a significant predictor of disease progression. In contrast, in stage II patients, the expression of CCL5 (CCL5 + ), the absence of ER-a (ER-a), and the lack of PR expression (PR) increased significantly the risk for disease progression (P = 0.0045, 0.0041, and 0.0107, respectively). The prognostic strength of CCL5, as well as of ER-a ,i mproved by combining them together (CCL5 + /ER-a: P = 0.0001), being highly evident in the stage IIA subgroup (CCL5 + /ER-a (P =0 .0003); ER-a (P = 0.0315)). In the stage II group as a whole, the combinations of CCL5/ER-a + and CCL5/PR + were highly correlated with an improved prognosis. Multivariate analysis indicated that, in stage II patients, ER-a and CCL5 were indepen- dent predictors of disease progression. Conclusions: CCL5 could be considered as a biomarker for disease progression in stage II breast cancer patients, with the CCL5 + /ER-a combination providing improved prediction of disease