Protective effect of hydrogen rich saline solution on experimental ovarian ischemia reperfusion model in rats

Abstract Background The present study aimed to investigate the effects of hydrogen rich saline solution (HRSS) in a rat model of ovarian ischemia–reperfusion injury. Methods Thirty-six female Wistar-albino rats were grouped randomly, into six groups of six rats. The groups were classified as: sham (S), hydrogen (H), torsion (T), torsion/detorsion (TD), hydrogen-torsion (HT), and hydrogen-torsion/detorsion (HTD). Bilateral adnexal torsion was performed for 3h in all torsion groups. HRSS was given 5ml/kg in hydrogen groups intraperitoneally. Malondialdehyde (MDA) and glutathione- S -transferase (GST) levels were measured in both the plasma and tissue samples. Tissue sections were evaluated histopathologically, and the apoptotic index was detected by TUNEL assay. The results were analyzed by Kruskal–Wallis and Pearson chi-square tests using computer software, SPSS Version 20.0 for Windows. Results The MDA levels were higher and GST levels were lower in the torsion and detorsion groups when compared to other groups, but the differences were insignificant ( P >0.05). The MDA levels were lower and GST levels were higher in the HT and HTD groups compared with the T and TD groups ( P >0.05). Follicular injury, edema, vascular congestion, loss of cohesion and apoptotic index were higher in the torsion groups but decreased in the groups that received HRSS. Conclusions According to histopathological and biochemical examinations, HRSS is effective in attenuating ischemia–reperfusion induced ovary injury.