Synthesis and characterization of PANI-Fe x -Al 1 − x ( x = 0.8, 0.6) nanocomposite thin films for identification of pathogenic Leptospira

Polyaniline Fe-Al nanocomposite thin films were fabricated on a glass substrate using a spin coating technique. The detection and identification of pathogenic Leptospira by measuring a current-voltage (I-V) and electrochemical impedance spectroscopy (EIS) showed a relationship of concentration bacteria vs. current and vs. impedance. Structural properties and morphology of nanocomposites were evaluated using energy-dispersive X-ray spectroscopy (EDX), atomic force microscope (AFM), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FESEM). TEM and FESEM images showed the uniformed distribution of nanoparticles of Fe-Al alloy. The AFM images showed the roughness of the thin film surface to perform at a higher sensitivity. The EDX analysis confirms the presence of the polyaniline, Fe, and Al elements in the substrate. This bacterial biosensor has also been tested with a bacteria concentration, ranging from 1 × 105 to 1 × 108 CFU mL−1, in wet samples for disease preventions.