A systematic review and meta-analysis of DNA methylation levels and imprinting disorders in children conceived by IVF/ICSI compared with children conceived spontaneously

† Introduction † Methods Population, exposures and comparators Outcome measures Literature searches Inclusion criteria Exclusion criteria Types of tissue samples included in the review Selection of studies, data extraction and meta-analysis Risk of bias (quality of the included studies) † Results Epigenetic and imprinting disorders in children conceived through IVF/ICSI compared with children conceived spontaneously DNA methylation at specific regions Overall risk of imprinting disorders in children conceived through IVF/ICSI compared with children conceived spontaneously DNAmethylation levels and the riskof epigenetic and imprinting disorders in childrenborn spontaneously to infertile couples compared with children born spontaneously to fertile couples † Discussion Principal findings Strengths and weaknesses Comparison with the literature Meaning of the study † Conclusion Study group Sample collection Laboratory analysis Data reporting