Enzymatic Preparation of Alkanedicarboxylic Acid Monoesters

Hydrolysis of alkanedicarboxylic acid dimethyl esters using esterase from Pseudomonas putida MR-2068 gave exclusively pure monoesters. Hydrolytic activities were dependent on the carbon chain length of the substrates. This esterase also catalysed enantio- and regio-selective hydrolysis of α-methylalkanedicarboxylic acid dimethyl esters.