Colony-live — a high-throughput method for measuring microbial colony growth kinetics — reveals diverse growth effects of gene knockouts in Escherichia coli

Background Precise quantitative growth measurements and detection of small growth changes in high-throughput manner is essential for fundamental studies of bacterial cell. However, an inherent tradeoff for measurement quality in high-throughput methods sacrifices some measurement quality. A key challenge has been how to enhance measurement quality without sacrificing throughput.