Evaluation of the Enzymatic Assay of Serum Uric Acid with 2,2′-Azino-Di(3-Ethylbenzthiazoline-6-Sulphonate) (ABTS) as Chromogen

A spectrophotometric method for the determination of serum uric acid based on the oxidation of 2,2′-azino-di(3-ethylbenzthiazoline-6-sulphonate) by use of uricase and peroxidase has already been reported. The method is very precise (CV <4·7%). The standard curve is linear up to 4640 μmol/L. Comparison with other enzymatic methods gave good correlation. The method gave results 9% lower than the phosphotungstate method. The effects of bilirubin, haemoglobin, glucose, ascorbic acid, anticoagulants and purine compounds were studied. The reference values for this method are 140·8–407·8 μmol/L for female subjects and 145·6–514·7 μmol/L for male subjects.