Influence of β-NGF on motile and invasive ability of human pancreatic cancer cell line MIA PaCa-II

OBJECTIVE:To investigate the roles of β-NGF on motile and invasive ability of human pancreatic cancer cell line MIA PaCa-Ⅱ,and its mechanism.METHODS:β-NGF was added to cell culture medium.Then the motile ability was tested by the crossing-river test and transwell migration assay,the invasive ability was evaluated by the matrigel invasion method,and the expressions of E-cadherin and MMP-2 were detected by Western Blot,respectively.RESULTS:The motile and invasive ability of MIA PaCa-Ⅱ obviously increased with the increase of β-NGF concentrations in cell culture medium.There were statistical differences of crossing-river time between β-NGF group(42.50±4.12) h and control group(68.45±3.53) h.By matrigel invasion test,the penetrated cells were(32.61±3.85)/field,(39.35±5.24)/field,(47.90±6.82)/field in 50,100 and 150 μg/L β-NGF groups,and(23.90±4.56)/field in control group,respectively,P0.01.The expression of E-cadherin significantly increased,and MMP-2 decreased in β-NGF groups.CONCLUSION:β-NGF might play potentia1 roles in invasion and metastasis of pancreatic cancer,by the down regulation of E-cadherin and up regulation of MMP-23.