Induction of CAD gene amplification by restriction endonucleases in V79,B7 Chinese hamster cells

Abstract The restriction endonucleases PvuII, BamHI and EcoRI were tested for their ability to induce gene amplification in V79,B7 Chinese hamster cells. The results indicate that treatment with these enzymes efficiently increases the frequency of clones resistant to N -phosphonacetyl- l -aspartate, indicating induction of CAD gene amplification.