Characterisation of potential probiotic Lactobacillus strains of human origin
2010
Molecular biological methods (genus-specific PCR,
species-specific PCRs, RAPD with primer M13, and rep-PCR with
primer (GTG)5) were applied for the identification and
discrimination of 31 strains of the Lactobacillus genus
isolated from faecal samples of 30 breastfed infants. All
strains were tested for bile salts and low pH tolerance, growth
in milk, production of lactic acids in milk and milk
supplemented with yeast extract, and used in biochemical tests
including production of beta-glucuronidase. Consequently, the
presence of genes coding for decarboxylases was investigated
using PCR. Finally, four strains of probiotic species were
selected for use in the dairy industry and for other tests
concerning their probiotic character.
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