Prokaryotic Expression of HLA-B*2705 Heavy Chain and Identification for Its Activity

In order to investigate the expression of heavy chain of HLA-B*2705 in prokaryotic system and identify its activity, the extra-membrane gene fragment of HLA-B*2705 was amplified from full-length HLA-B*2705 cDNA by PCR and cloned into pGEM-T vector. After identification by sequencing, the prokaryotic expressing vector pET32a(+)-B*2705 was constructed. The antigenic activity of expressed protein was identified by Western blot and antibody blocking reaction. The results indicated that the fused HLA-B*2705 protein expression with high efficiency was obtained. The expressed product was more than 50% of the total bacteria protein. The antigenic activity of expressed protein was confirmed by Western blot and antibody blocking reaction. It is concluded that HLA-B*2705 fusion protein are obtained as basis for the further studies.