Raman Spectroscopic Analysis of Single Red Blood Cells in Vivo Rat

Raman spectroscopy was used to study red blood cells (RBCs) and blood in vein capillary, in artery capillary in vivo and in vitro with the use of optical tweezers. Results showed that, (1) We could get Raman spectroscopy of blood in vivo; (2) We could clearly observe that different blood spectra in. oxygenated and deoxygenated state in artery and vein capillary; (3) By studying Raman spectroscopy of single RBC, we concluded that the hemoglobin concentration in capillary was larger than that in vitro, and oxygenated Raman peak of RBC in artery capillary was More obvious than those in vein capillary; (4) By comparing Raman spectrum in vein with that in artery, we found that that pH value in artery capillary is higher than that in vein capillary, and by studying the change of the band at 1604 cm(-1), we found that the band change was in connection with the circumstance pH value around RBCs. These findings make it possible to study conformational changes within single cells in vivo. The experiments also have shown that laser tweezers Raman spectroscopy can provide a strong tool for research in vivo.