A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and DNA markers for selecting the plant of said raphanus sativus line

The present invention relates to a new type Cytoplasmic Sterility type (the CGMS) and a radish plant-based DNA markers for breeding the plants, more precisely, with new CGMS (D-CGMS) based Radish plants for hybrid seed production methods the present invention further relates to the above-mentioned plants, chloroplast DNA markers, SNP markers method of hybrid seed production based on mitochondrial DNA, wherein the D-CGMS for breeding based radish plants with chloroplast DNA marker SEQ.ID .NO: nucleotide sequence shown Breeding 5, based on mitochondrial DNA of the SNP (single nucleotide polymorphism) markers located SEQ.ID.NO Breeding D-CGMS based radish plants: Breeding 171 nucleotides of the nucleotide sequence shown in Figure 12. D-CGMS based radish plant of the invention, due to the high stability of male sterility, can be effectively used for the production of hybrid seed. Chloroplast DNA based markers based SNP markers and mitochondrial DNA, in the breeding process can be effectively breeding D-CGMS radish plant breeding D-CGMS system based Radish plants.