The Role of Anti-Dectin-1 Monoclonal Antibody in the Production of TNF-α and NO by Murine Macrophage Stimulated by Candida Albicans

Objective To investigate the role of anti-Dectin-1 monoclonal antibody in the production of tumor necrosis factor-alpha (TNF-α) and Nitric Oxide (NO) by murine macrophage stimulated with Candida albicans.Methods ①Mouse peritoneal macrophages were preincubated with anti-Dectin-1 monoclonal antibodies in different concentrations in disposal groups;while in negative control groups,an isotype-matched control monoclonal antibodies was used.②TNF-α concentrations in the supernatant of each group were measured by enzyme-linked immunosorbent assay after stimulated with heat-killed or live Candida albicans for 1h,3h,6h,8h.③The NO level in the supernatant were determined by Griess reaction after stimulated with heat-killed or live Candida albicans for 6h.Results ①The TNF-α concentration was significant(79.82±11.74pg/mL)lower than that of the negative control group(105.16±12.36pg/mL)after stimulated for 1h in the disposal group of 20μg/mL anti-Dectin-1 monoclonal antibodies (P0.05).However,after stimulated for 3h,6h,8h,the TNF-α concentrations in the supernatant of the disposal groups were much lower than that of the negative control groups(P0.01).The NO concentrations of the disposal groups(61.24±8.85μmol/L,45.36±3.92μmol/L,36.37±2.58μmol/L)were lower than that of the negative control groups(87.65±9.17μmol/mL)after stimulated for 6h (P0.05).Conclusion Anti-Dectin-1 monoclonal antibody plays an inhibitory role in the production of TNF-α and NO by murine macrophage after stimulated with Candida albicans.