Force Spectroscopy with Quantitative On-Cantilever Force Control

In this work we propose a method for on-cantilever force control for application in force distance (F-z) spectroscopy for intermolecular interaction sensing. In this method we perform the F-z measurements with cantilevers integrating functionalized spheres attached to them. The F-z curve is obtained by controlling the Lorentz force acting at the cantilever free end. The intermolecular interaction forces are measured by measuring the force necessary to retract the cantilever from the surface. The required force is generated by the current passing through the cantilever. In this scenario we monitor the bending of cantilevers using optical beam deflection (OBD) method. Moreover, as deflection of the cantilever was electromagnetically controlled it was possible to calibrate the OBD response as well.