The Effects of Amniotic Membrane Ointment on Corneal Alkali Burn in Rabbits

Purpose : The therapeutic effects of amniotic membrane transplantation (AMT) contain anti-fibrosis, anti-inflammation and facilitation of epithelialization. Thus we investigated whether applying of amniotic membrane (AM) ointment could get the same effect as AMT. Methods : Alkali burn was induced by applying 6 mm round filter paper which was soaked with 1 N NaOH, onto the central cornea for 60 seconds on both eyes of 10 white rabbits. Then we applied AM ointment on one eye and base ointment on the other eye, four times a day for 1 week. The corneas were harvested for histopathologic studies at 1 day and 3 weeks. Corneal opacity and thickness were measured in 3 days and 1, 2, 3 weeks. Results : In the AM ointment applicated group, the number of the infiltrated PMNs (polymorphonuclear cells) and apoptotic keratocytes were significantly less than that of the control group (P