A DYNAMICAL PROFILE OF METAL INCORPORATION TO A LIPOSOMAL MEMBRANE AS STUDIED BY ELECTRON SPIN RESONANCE

An Egg-Yolk lecithin liposome containing the surfacant cyclic polyamine was prepared, and a dynamical behavior of its Cu(II) ion uptake was studied by monitoring a time dependent decay of the isotropic copper nucleus hyperfine splitting in the ESR upon metal incorporation into a liposomal membrane. The reaction kinetics studied by ESR justified the fact that there are the two kinds of metal binding processes, in which the distinctive time course and the activation energy can be clearly demonstrated.