[Study on purification of polysaccharides in root of Salvia miltiorrhiza by macroporous adsorption resin and ion-exchange resin methods].

Objective:To study the purification and isolation of polysaccharides from Salvia miltiorrhiza.Methods: The root was extracted by water purified preliminarily by alcohol precipitation,and then four different types macroporous adsorption resin and one ion-exchange resin were comparatively investigated in the purification and isolation of Salvia polysaccharides.Results: The total polysaccharides of crude extracts was 40.35%,and protein content amounted to 8.96%.Compared with the traditional methods,AB-8,DB-301 type of resin used in purification of polysaccharides could simplify the working process and obtain better effect.Then the obtained crude polysaccharides through AB-8 resin were purified by ion-exchange resin DEAE-52.Three portions of powder were obtained through lyophilization and named as SMP1,SMP2,SMP3.Conclusion: Purification of Salvia polysaccharides can be conducted by adoping AB-8,DB-301 type of resin and DEAE-52 ion-exchange resin.