Bioprospecting of Ocotea minarum (Laurales: Lauraceae) by ethanolic extract in control of strains of gender Candida
2014
into 100 mL of microdilution plate with 96 wells. Immediately after it was added 100 mL of inoculum at a concentration of 0.5 McFarland (108 CFU / ml) in the wells. The microbial suspension was used as positive control, while broth containing the extract as a negative control was used, MIC values were analyzed with the lowest concentration of the extract in the wells of microdilution plate showed no turgor after inoculation. Samples were removed from each well of the plate microdilution MIC and perforated in a petri dish containing Sabouraud Dextrose agar (Difco) for evaluating the minimum fungicidal concentration (MFC) [4]. Results and conclusions Based on the criteria of Araujo [5], the evaluation of the antifungal activity of ethanol extract of Ocotea minarum, observed effective antifungal activity against the strains of Candida tropicalis in a concentration of 64 mg/mL and Candida krusei concentration of 1024 mg/ml. Strains of Candida albicans and Candida glabrata were not growth inhibited by the extract. With these results we can conclude that the ethanol extract of the bark of Ocotea minarum studied shows antifungal activity and therapeutic potential feasible and cost effective. The results were obtained by in vitro studies, aiming later conducting research in vivo, so that this extract can be used to obtain a bioactive principle in the production of pharmaceuticals and cosmetics, aimed at curing diseases clinics related to the genus Candida.
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