Xenotransplantation of human cultured parathyroid progenitor cells into mouse peritoneum does not induce rejection reaction.

Introduction: Parathyroid progenitor cells devoid of immunogenic antigens were used for human allotransplantation. although there were many potential reasons for the expiry of transplant activity in humans, we decided to exclude a subclinical form of rejection reaction, and test the rejection reaction in an animal model. Material and methods: Experiments were carried out on 40 conventional male mice in their third month of life. the animals were housed in groups of 10 per cage in 4 cages with fitted water dispensers and fed a conventional diet based on standard pellet food. they were divided into four groups of 10 animals each, three experimental groups and one control group. Identified progenitor cells were stored in a cell bank. after testing the phenotype, viability, and absence of immunogenic properties, the cells were transplanted into mouse peritoneum cavity. Results: animals were observed for 9 weeks. at 9 weeks of observation, the mean serum Pth concentration in the experimental groups was 2.0-2.5 pg/ml, while in the control group it did not exceed 1.5 pg/ml. the immunohistochemical assays demonstrated that millions of viable cells with a phenotype identical to the endocrine cells had survived in the peritoneum. histologic specimens from different internal organs stained for Pth revealed positive cells labelled with anti-Pth ab in the intestinal lamina, brain, liver, and spleen. Conclusions: In the present paper we have demonstrated that xenotransplantation may be used as a model for an explanation of the immunogenic properties of cells generated from postnatal organs for regenerative therapy.