Antioxidant Activity and Characterization of Exiguobacterium sp. SC2-1 Isolated from Sea Water

For the reseach of the natural marine antioxidant, an antioxidant-producing bacterium was isolated from seawater in Jeju costal area. The isolated strain SC2-1 was Gram-positive, catalase positive, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and NaCl required for growth. The radical scavenging activity of the culture supernatant was detemined by DPPH method. This bacterium was identified based on morphological, biochemical characteristics and 16S rDNA sequence analysis, and then was named Exiguobacterium sp. SC2-1. The optimum conditions of culture for production antioxidnat were , pH 6-8 and NaCl. The stain showed the highest activity and growth cultured in medium which added maltose. Hydroxyl radical activity of the supernatant of Exiguobacterium sp. SC2-1 was . The SOD activity of the culture supernatant was estimated about .