Time Course of Oxidative Stress Status in the Postprandial and Postabsorptive States in Type 1 Diabetes Mellitus: Relationship to Glucose and Lipid Changes

Objective: The aim of this study was to compare oxidative stress status (OSS) with blood glucose and lipid changes during the fasting, postprandial and postabsorptive phases in type 1 diabetes mellitus.Methods: Twenty-three patients on intensive insulin treatment received a standard fat-rich breakfast and lunch. OSS was monitored at fasting (F), just after the post-breakfast glycemia peak (BP) (identified by continuous subcutaneous glucose monitoring), 3.5-h post-breakfast (B3.5), just after the post-lunch peak (LP), just after the post-lunch dale (LD) and 5 hours after lunch (L5).Results: Whereas whole blood glutathione and plasma protein thiols increased in the postprandial period (from 6.52 ± 1.20 (F) to 7.08 ± 1.45 μmol/g Hb (BP), p = 0.005), ascorbate decreased gradually from 44 ± 17 (F) to 39 ± 19 μmol/L (LD), p = 0.015. Retinol and α-tocopherol also decreased from 27.1 ± 7.0 (F) to 25.3 ± 5.2 μmol/L (BP), p = 0.005. Uric acid decreased later, from 213 ± 77 (BP) to 204 ± 68 μmol/L (B3.5), p = 0.01, ...