Geminivirus en Tomate (Lycopersicon esculentum Mill.) y Rango de Hospedantes en Baja California Sur, México

Three uncharacterized geminiviruses belonging to the genus Begomovirus were detected in tomato plants in the State of South Baja California (SBC), Mexico, using molecular techniques. General detection was achieved by DNA hybridization with a PHV-A probe. PCR reactions were performed with degenerated primers targeting the coat protein gene and the common or intergenic region. PCR products were cloned, sequenced and compared by local alignment (BLAST) using sequences of reference geminiviruses from the GenBank database (NCBI). Sequences that yielded the highest scores were compared by multiple alignment (MegAlign, DNASTAR). Sequences from three different isolates (LaPS, LaPCh, LaPU3) from the area of La Paz, and one isolate from El Carrizal (CzalB-d21) were thus analyzed. LaPS shared a 93% sequence identity to Tomato severe leaf curl virus (ToSLCV); LaPCh 95% to a partially characterized geminivirus, tentatively named as Tomato mild mottle virus (TMMV); LaPU3 98% to Pepper golden mosaic virus (Recibido: Agosto 7, 2003 Aceptado: Noviembre 11, 2003) Revista Mexicana de FITOPATOLOGIA/ 107