Electrochemical immunoassay method for bisphenol A detection

The invention provides an electrochemical immunoassay method for bisphenol A detection. The method comprises the steps of 1, competing reaction, wherein 5-20 microlitres of iso-volume mixed gradient dilution type solution of a sample to be detected or sample extraction solution and enzyme-labelled antigen solution diluted by phosphate buffered solution are added onto the surface of an enclosed electrode dropwise, the enclosed electrode is placed at 20-35 DEG C for incubation lasting 0.5-1.5 h, and flushing and blow-drying are conducted; 2, reduction peak current value detection, wherein the flushed electrode obtained from step 1 is immersed in 2-6 millilitre of ferrocenylmethanol solution of 0.2-1.5 mM, 20-60 microlitres of hydrogen peroxide of 5-15 mM is added at the same time, and then reduction peak current value is detected with the cyclic voltammetry method with a saturated calomel electrode as the reference electrode and a platinum electrode as the counter electrode. The immunoassay method is used for detection. The method overcomes the defect of a traditional enzyme-linked immunoassay method that detection is time-consuming, and reduces detection time.