Research of irradiation of high—power He—Ne laser on hypertrophic scar in rabbits

Objective To explore the therapeutic effects and mechanisms of high power He Ne laser irradiation on hypertrophic scar(HS) in rabbits by setting up hypertrophic scar animal model.Methods 32 round wounds(diameter of 6 mm) and skin layer loss were made on ventral side of rabbits' ears using a trephine.21 days after the operation,10 projecting scars were irradiated with He Ne laser for 30 minutes every other day,for two weeks.Another 10 projecting scars were as control group.35 days later,cell apoptosis rate and PCNA protein expression of the scar histology were measured by TUNEL technique and immunohistochemistry method,respectively.Results In He Ne laser group,scars became much thinner than those in control group(P 0.05).Cell apoptosis rate increased greatly while PCNA protein expression decreased a lot.There were significant difference between the two groups(both P 0.05).Conclusion It shows that high power He Ne laser could inhibit the growth of HS in rabbits by inducing cell apoptosis and inhibiting cell proliferation probable.